The nucleotide-binding site ofAquifex aeolicusLpxC
نویسندگان
چکیده
منابع مشابه
آنالیز پروفایل nbs (nucleotide binding-site) ژنهای مقاومت به بیماری در ارقام مختلف زیتون
به منظور مطالعه ژنهای مقاومت nbs-lrr و تنوع ژنتیکی آنها در بین ارقام بومی زیتون و همچنین مقایسه آن با ارقام خارجی، از تکنیک profiling nbs و آنالیزهای مولکولی استفاده شد. برای این منظور dna ژنومی تعداد 5 رقم خارجی و 5 رقم داخلی زیتون استخراج و با آنزیم های برشی alui و rsai هضم و با استفاده از 3 آغازگر دژنره( nbs2،nbs7،nbs5a) در دو مرحله تکثیر شدند و الگوی باندی حاصل از تکثیر انتخابی آنها در روی ...
The nucleotide-binding site of human sphingosine kinase 1.
Sphingosine kinase catalyzes the formation of sphingosine 1-phosphate, a lipid second messenger that has been implicated in a number of agonist-driven cellular responses including mitogenesis, anti-apoptosis, and expression of inflammatory molecules. Despite the importance of sphingosine kinase, very little is known regarding its structure or mechanism of catalysis. Moreover, sphingosine kinase...
متن کاملThe nucleotide-binding site of Aquifex aeolicus LpxC
The structure of recombinant Aquifex aeolicus UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) in complex with UDP has been determined to a resolution of 2.2 A. Previous studies have characterized the binding sites of the fatty-acid and sugar moieties of the substrate, UDP-(3-O-hydroxymyristoyl)-N-acetylglucosamine, but not that of the nucleotide. The uracil-binding site is constructed from ...
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HIV-1 integrase is essential for viral replication and can be inhibited by antiviral nucleotides. Photoaffinity labeling with the 3'-azido-3'-deoxythymidine (AZT) analog 3',5-diazido-2', 3'-dideoxyuridine 5'-monophosphate (5N3-AZTMP) and proteolytic mapping identified the amino acid 153-167 region of integrase as the site of photocrosslinking. Docking of 5N3-AZTMP revealed the possibility for s...
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Comparative molecular field analysis (CoMFA) predicts that the large electrostatic field around the phosphate groups of ATP plays a crucial role in stabilizing the open state of the cardiac ryanodine receptor (RyR) channel. We therefore investigated the effects of adenosine-5'-(beta,gamma-methylenetriphosphate) (AMP-PCP), an ATP analog with lower negative charge in this region, on the gating of...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2006
ISSN: 1744-3091
DOI: 10.1107/s1744309106041893